Main Article Content
Abstract
Protein denaturation, one of the intricate biological processes involved in inflammation, leads to tissue damage and inflammatory diseases. Using the egg albumin protein denaturation assay, the current study assessed the ethanolic seed extract of Caesalpinia crista's in vitro anti-inflammatory effectiveness. Following Soxhlet extraction of the seeds, flavonoids, alkaloids, tannins, saponins, and other secondary metabolites were detected by first phytochemical screening. Indomethacin was utilized as the reference medication, and anti-inflammatory activity was evaluated at several doses (20–100 µg/mL). With a maximal inhibition of 90.47%, the ethanolic extract demonstrated concentration-dependent inhibition of protein denaturation, demonstrating notable efficacy at higher doses. The extract showed noteworthy protein stabilizing characteristics, while having a somewhat lower activity than indomethacin. The presence of bioactive phytoconstituents may be responsible for the effect that has been seen. These results imply that Caesalpinia crista seeds have encouraging anti-inflammatory properties and merit additional mechanistic and in vivo research.